Friday, November 8, 2019

PRIME EDITING, A PROMISING NEW TOOL


Linda and her Mom
Before I came along

Well, by this time you know all about the more popular twists and turns of immunotherapy:  PARP inhibitors, CRISPR-Cas9, tumor-infiltrating lymphocytes – that sort of thing.  If you are at all like me, you have a vague idea of what they do – but are completely mystified by how they do it.  Well, here is another mystery to add to your list – prime editing.  It’s all the buzz.

Seems that folks at the Broad Institute of MIT, and other folks at Harvard, have devised a precise and delicate way to edit the genome.  Unlike previous CRISPR techniques, which were good for destroying genes, prime editing enables the investigator to fix them, by engineering  a swap of a single base – T for A, say, or A for C, etc.  There are 12 possible ways that genomic coupling can go wrong, and prime editing can fix them all.  Where CRISPR Cas9 is a chain saw, prime editing is a pair of tweezers.

This work has been published in Nature very recently and has created a stir.  There are articles discussing it everywhere you turn.  The link given below is easy going.  I am planning to read around and, hopefully, figure out how prime editing actually works.  Stay tuned.  But, in the meantime Google "prime editing", and see what you find.




1 comment:

  1. I doubt that NIH Director Francis Collins could write a dull word if he tried. Here he is explaining prime editing and its significance. Well worth reading.

    https://directorsblog.nih.gov/2019/11/05/gene-editing-advance-puts-more-gene-based-cures-within-reach/

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